Expanding the scale and scope of the Marine Biodiversity Observation Network Pole to Pole of the Americas: Merging rocky intertidal biodiversity surveys with environmental DNA and plankton imaging applications

The Marine Biodiversity Observation Network Pole to Pole of the Americas (MBON Pole to Pole) brought together 30 participants from 10 countries in Patagonia, Argentina, to strengthen observing capacity of coastal biodiversity across the Americas. The network held a five-day workshop focused on three core components: standardized rocky intertidal photo-quadrat surveys, low-cost environmental DNA (eDNA) sampling, and affordable plankton imaging tools. Participants included researchers, park rangers, and conservation practitioners fostering a collaborative and inclusive environment. Key outcomes included field validation of protocols, identification of context-specific methodological adaptations (e.g., for low tidal amplitude areas), adoption of novel tools for monitoring marine life, and strategies for broader participation and data harmonization. The workshop highlighted the potential of simple, replicable methods to support long-term monitoring, and emphasized the value of shared protocols, tools, and open data for building a more connected and resilient regional observation network.